Genomics

Dataset Information

235

A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium


ABSTRACT: A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium

PROVIDER: PRJNA208369 | ENA |

REPOSITORIES: ENA

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A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium.

Nature biotechnology 20140824 9


We present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference RNA samples with built-in controls, we assess RNA sequencing (RNA-seq) performance for junction discovery and differential expression profiling and compare it to microarray and quantitative PCR (qPCR) data using complementary metrics. At all sequenci  ...[more]

Publication: 1/2

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