Genomics

Dataset Information

118

Homo sapiens


ABSTRACT: Impact of library preparation on downstream analysis and interpretation of RNA-seq data: comparison between Illumina PolyA and NuGEN Ovation protocol

ORGANISM(S): Homo sapiens  

PROVIDER: PRJNA208380 | ENA |

REPOSITORIES: ENA

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Publications

Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol.

Sun Zhifu Z   Asmann Yan W YW   Nair Asha A   Zhang Yuji Y   Wang Liguo L   Kalari Krishna R KR   Bhagwate Aditya V AV   Baker Tiffany R TR   Carr Jennifer M JM   Kocher Jean-Pierre A JP   Perez Edith A EA   Thompson E Aubrey EA  

PloS one 20130819 8


OBJECTIVES: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses of the broad applications of RNA sequencing. METHODS AND MATERIALS: Eight human mammary epithelial cell (HMEC) lines with high quality RNA were sequenced by Illumina's mRNA-Seq PolyA selection and NuG  ...[more]

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