Project description:To investigate the function of NCOR2-013 in T. marneffei-infected THP-1 macrophages, we established NCOR2-013 overexpression THP-1 macrphages. We then performed gene expression profiling analysis using data obtained from RNA-seq of T. marneffei-infected NCOR2-013 overexpression THP-1 macrophages and T. marneffei-infected control cells.
Project description:Comparative analysis of transcriptome of Penicillium marneffei PM1 grown at 25°C and 37°C Penicillium marneffei strain PM1 was pre-cultured at 25°C and 37°C for two weeks on Sabouraud's Dextrose Agar (SDA). Messenger RNAs were then isolated from one-week 25°C and 37°C cultures and sequenced with Illumina by BGI America. Two replicates.
Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25°C or a unicellular fission yeast form at 37°C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are ‘phase or cell-state specific’ transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types.
