Verticillium alfalfae
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ABSTRACT:
PROVIDER: PRJNA212808 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Pathogenic fungus that causes Verticillium wilt disease in plants
| PRJNA28533 | ENA
Project description:Verticillium dahliae is a soilborne fungus that causes wilt disease in plants. The microsclerotia of V. dahliae produce infectious hyphae that give rise to primary infections. In this study, RNA-seq libraries were prepared from microsclerotia (MS)-producing cultures of V. dahliae (ave = 52.23 million reads), and those not producing microsclerotia (NoMS, ave = 50.58 million reads) and analyzed for differential gene expression.
2013-09-06 | E-MTAB-1581 | biostudies-arrayexpress
Project description:The soilborne fungus, Verticillium dahliae, causes Verticillium wilt disease in plants. Verticillium wilt is difficult to control since V. dahliae is capable of persisting in the soil for 10 to15 years as melanized microsclerotia, rendering crop rotation strategies for disease control ineffective. Microsclerotia of V. dahliae overwinter and germinate to produce infectious hyphae that give rise to primary infections. Consequentially, microsclerotia formation, maintenance, and germination are critically important processes in the disease cycle of V. dahliae.
2013-09-06 | E-MTAB-1793 | biostudies-arrayexpress
Project description:Cotton is the main source of natural fiber in the textile industry, making it one of the most economically important fiber crops in the world. Verticillium wilt, caused by the pathogenic fungus Verticillium dahlia, is one of the most damaging biotic factors limiting cotton production. Mechanistic details of cotton defense responses to verticillium wilt remain unclear. In this study, GFP-labeled strain of V. dahlia was used to track colonization in cotton roots, and clear conidial germination could be observed at 48 hours post-inoculation (hpi), marking this as a crucial time point during infection. Transcriptome analysis identified 1,523 and 8,270 differentially expressed genes (DEGs) at 24 hpi and 48 hpi, respectively. Metabolomic screening found 78 differentially accumulated metabolites (DAMs) at 48 hpi. Conjoint analysis indicated that the phenylpropanoid biosynthesis pathway was activated in cotton infected with V. dahliae. The five metabolites in the phenylpropanoid biosynthesis pathway, including caffeic acid, coniferyl alcohol, coniferin, scopoletin and scopolin, could significantly inhibit V. dahlia growth in vitro, implicating their roles in cotton resistance to Verticillium wilt. The findings expand our understanding of molecular mechanisms underlying the pathogen defense response against V. dahlia infection in upland cotton, which may lead to future insights into controlling Verticillium wilt disease.
2023-12-26 | GSE221845 | GEO
Project description:To verify the pathogenicity of Lecanicillium psalliotae invasive pathogens on tsearch leaves, Lecanicillium psalliotae was identified by isolation and purification. sweet orange leaves were infested with it. The results of the experiments showed that 15 days after Lecanicillium psalliotae infested the leaves of sweet orange, yellow spots grew around the pores and irregular yellow spots appeared on both sides of the leaf veins. This was highly similar to the disease in the field, suggesting that Lecanicillium psalliotae is the causal agent of the yellow spots on sweet orange leaves that cause the leaves to wilt and fall off. In previous studies, Verticillium cutaneum was mainly identified as a biological control agent and a suspected pathogen. In this study, the pathogenicity of Verticillium cutaneum was verified for the first time as a causal agent of leaf spot disease of plants.
2023-09-09 | GSE234373 | GEO
Project description:Verticillium dahliae is a soil-borne vascular pathogen that causes severe wilt symptoms in a wide range of plants. Co-culture of the fungus with Arabidopsis roots for 24 hours induces many changes in the gene expression profiles of both partners, even before defense-related phytohormone levels are induced in the plant. Both partners reprogram sugar and amino acid metabolism, activate genes for signal perception and transduction, and induce defense and stress responsive genes. Furthermore, analysis of Arabidopsis expression profiles suggests a redirection from growth to defense. The plant and fungal genes that rapidly respond to the presence of the partner might be crucial for early recognition steps and the future development of the interaction. Thus they are potential targets for the control of V. dahliae-induced wilt diseases.
2018-02-08 | GSE104590 | GEO
Project description:Verticillium alfalfae VaMs.102 Genome sequencing and assembly
| PRJNA29511 | ENA
Project description:The soil-borne ascomycete Verticillium dahliae which causes wilt disease in many important crops results a severe reduction in crop yield and quality. During infection, the V.dahliae will secrete a lot of secondary metabolites which act as toxic factors to promote the infection process. However, the mechanism underlying how V.dahliae secondary metabolites regulate cotton infection remains largely unexplored. BRE1 is a highly conserved ubiquitin ligase (E3) enzymes and regulate expression of specific genes related to the production of secondary metabolites. To get an insight into the genes regulated by VdBre1, the comparative transcriptomic analysis was conducted between VdBre1 deletion mutant and the wild type V592. The conidia of wilde type and mutant were collected and total RNA was extracted using fungal RNA kit, sequencing was performed on an BGISEQ-500 platform.
2021-05-20 | GSE151401 | GEO
Project description:Plant roots secrete secondary metabolites to sense the enviroment around them. Among them, terpenes play a prominent role. Terpenes can have either fungistatic or fungicide action. However, their exact role in plant-host interactions is not fully understood. Verticillium longisporum is a soilborne pathogen causing disease in Brasicacae plants. In this project we investigated the transcriptomic changes of this species upon exposure to the β-pinene monoterpene in different time points 0hpi, 8hpi, 24hpi and 24hpi.
2021-11-07 | GSE158956 | GEO
Project description:Verticillium wilt of potato caused by Verticillium dahliae, also called potato early dying disease, is an important factor limiting potato production in the world. Currently, the poor understanding of the pathogenic mechanism of Verticillium dahliae has led to increasingly severe challenges in the effective control of Verticillium wilt. Many studies have shown that pigment biosynthesis related proteins are virulence factors of Verticillium dahliae, while the regulatory mechanisms are still unknown. In order to elucidate the pathogenic mechanism regulated by the virulence related pigment biosynthesis protein Vayg1 in Verticillium dahliae, proteomic analysis will be used to judge the Vayg1 deletion mutant and the wild-type strain during the infection of potato to explore the Vayg1 involved pathogenic protein network, aiming to identify and understand relevant pathogenic pathways to provide an important support for deeper understanding of the pathogenic mechanism of the pathogen. The key proteins related to virulence and molecular networks that will be obtained in this study could provide new targets for the control of Verticillium wilt and new insights for the improvement of disease management.
2023-02-15 | PXD040131 | iProX