Project description:The Sordaria macrospora nox1 mutant has a block in sexual development at the stage of protoperithecia formation, and therefore is sterile. The aim of this study was to determine the transcriptome of microdissected protoperithecia from the mutant, as well as the transcriptomes of total RNA from the mutant and the wild type for comparison. The data were then compared with transcriptome data from protoperithecia of the wild type and the developemental mutant pro1; the corresponding data were generated in a previous study (Teichert et al. 2012, BMC Genomics 13: 511, GEO accession GSE33668). The samples (amplified RNA from nox1 microdissected protoperithecia and total RNA from nox1 and wild type mycelia undergoing sexual development) were sequenced as paired-end reads on an Illumina HiSeq 2000, two independent biological replicates for each nox1 sample and one replicate for the wild type.
Project description:The Sordaria macrospora nox1 mutant has a block in sexual development at the stage of protoperithecia formation, and therefore is sterile. The aim of this study was to determine the transcriptome of microdissected protoperithecia from the mutant, as well as the transcriptomes of total RNA from the mutant and the wild type for comparison. The data were then compared with transcriptome data from protoperithecia of the wild type and the developemental mutant pro1; the corresponding data were generated in a previous study (Teichert et al. 2012, BMC Genomics 13: 511, GEO accession GSE33668).
Project description:The aim of this analysis was to analyze DNA methylation in the wild type and the histone chaperone mutant asf1 of the filamentous fungus Sordaria macrospora.
Project description:The aim of this analysis was to analyze nucleosome distribution in the filamentous ascomycete Sordaria macrospora by micrococcal nuclease digestion and sequencing.
Project description:The aim of this analysis was to analyze gene expression in developmental mutants of the filamentous ascomycete Sordaria macrospora. RNA was isolated from total mycelia, or young fruiting bodies (protoperithecia) were isolated by laser microdissection and RNA was extracted, amplified and used for RNA-sequencing.
Project description:The aim of this analysis was to analyze gene expression in developing fruiting bodies (perithecia) of the filamentous ascomycete Sordaria macrospora, and to identify genes that are differentially regulated in the three developmental mutants delta-spt3, delta-asm2, and delta-asm3.
Project description:Chromatin organization of the wild type and delta-asf1 mutant of the filamentous ascomycete Sordaria macrospora was analyzed by Hi-C. The delta-asf1 mutant lacks the conserved histone chaperone ASF1 and is unable to produce sexual fruiting bodies. It was hypothesized that the lack of the histone chaperone ASF1 in the mutant might lead to differences in short- or long-distance interactions of chromatin. Furthermore, it was hypothesized that chromatin organization might change during sexual development in the wild type as a prerequisite for the drastic transcriptome changes during development that were observed in earlier studies.
