Project description:Purpose:The goals of this study are to clarify the B. subtilis NBRC 16449 response to soybeans. Methods: B. subtilis NBRC 16449 cells were aerobically cultured in liquid LB, LB solidified with agar, or on surface of boiled soybeans to logarithmic growth phase. Total RNAs were extracted from bacterial cells by Hot-Phenol method. Samples for RNA-seq were prepared according to Illmina protocol available from the manufacture. The sequence reads that passed quality filters were analyzed at the transcript isoform level with bowtie v0.11.2. Results: Using an optimized data analysis workflow, we mapped around 15 million sequence reads per sample to the whole genome of B. subtilis BEST195 and identified 4271 transcripts in B. subtilis NBRC 16449 with Bowtie aligner. Read count per genome was extracted from known gene annotations with HTSeq program. Compared the transcriptomes of B. subtilis NBRC 16449 grown on LB solidified with agar to that grown on surface of boiled soybeans, about 5% of genes showed the different expression levels.

2020-11-02 | GSE109523 | GEO

Lysinibacillus odysseyi

Project description:Lysinibacillus odysseyi overview

| PRJNA172815 | ENA

Expression analysis of Gluconacetobacter xylinus NBRC 3288 delta-fnrG mutant

Project description:Investigation of whole genome gene expression level changes in a Gluconacetobacter xylinus NBRC 3288 delta-fnrG mutant, compared to the wild-type strain.

2021-07-16 | GSE41775 | GEO

Bacillus smithii NBRC 15311

Project description:Bacillus smithii NBRC 15311 genome sequencing project

| PRJDB409 | ENA

Bacillus badius NBRC 15713

Project description:Bacillus badius NBRC 15713 genome sequencing project

| PRJDB1315 | ENA

Bacillus atrophaeus NBRC 15539

Project description:Bacillus atrophaeus NBRC 15539 genome sequencing project

| PRJDB406 | ENA

Bacillus pumilus strain:NBRC 12092_1 | isolate:NBRC 12092_1

Project description:Bacillus pumilus NBRC 12092_1 genome sequencing