Genomics

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RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome


ABSTRACT: RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome

PROVIDER: PRJNA215250 | ENA |

REPOSITORIES: ENA

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RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome.

Zwemer Lillian M LM   Hui Lisa L   Wick Heather C HC   Bianchi Diana W DW  

Prenatal diagnosis 20140629 10


The aim of this study was to compare the complexity of the amniotic fluid supernatant cell-free fetal transcriptome as described by RNA Sequencing (RNA-Seq) and gene expression microarrays.Cell-free fetal RNA from the amniotic fluid supernatant of five euploid mid-trimester samples was divided and prepared in tandem for analysis by either the Affymetrix HG-U133 Plus 2.0 Gene Chip microarray or Illumina HiSeq. Transcriptomes were assembled and compared on the basis of the presence of signal, rank  ...[more]

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