Project description:Transcriptional profile after growth in MM (minimal medium (Premaratne et al. 2001) supplemented with 50 mM Glucose. Note: strain "EGD" has not been deposited at ATCC/NCTC. It is a derivative of the original Murray strain (Murray et al. 1926), obtained from S.H.E. Kaufmann. Strain "EGD-e" has been obtained from H. Hahn (Berlin) via T. Chakraborty (Gießen) and is deposited under ATTC BAA-679 and in the Pasteur culture collection. Keywords: strain comparison
Project description:Bacteria were grown in BHI until OD600 1.0 (late exponential phase) and RNA stabilized using the RNAprotectTM bacteria reagent (QIAGEN) according to the manufacturer recommendations. For each strain, RNA extraction has been carried out on three independent biological samples. Briefly, bacterial total RNA was isolated, reverse transcribed, hybridised to whole genome microarray and microarray data was analysed as described previously (Chatterjee, 2006). Hybridizations were performed with cDNAs from EGD-e versus the over-expressing strain (EGD-e+pPXrnhB).
