Project description:ChIP-seq was conducted on isolated splenic WT CD8+ T cells, TCR-activated and cultured with IL-2 using anti-Runx3 antibodies (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3 and two NIS IP repeats from TCR-activated and IL-2 cultured splenic CD8+ T cells isolated by positive selection on anti-CD8 magnetic beads.

2013-11-18 | E-GEOD-50128 | biostudies-arrayexpress

Mus musculus

Project description:Runx3 function in splenic NK cells (IL-2 or IL-15)

| PRJNA216120 | ENA

E-GEOD-50121

Project description:Runx3 function in IL-2-activated splenic CD8+ T cells

2015-07-13 | E-GEOD-50121 | ExpressionAtlas

The interactomes of RUNX1 and RUNX3 from Mus musculus T cells

Project description:Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.

2019-11-12 | PXD007511 | Pride

Mus musculus

Project description:Genome-wide maps of Runx3 bound regions in splenic IL-2-activated CD8+ T cells

| PRJNA216113 | ENA

Mus musculus

Project description:Runx3 function in splenic NK cells

| PRJNA216119 | ENA

Mus musculus

Project description:Runx3 function in CD4+ splenic dendritic cells

| PRJNA210763 | ENA

Mus musculus

Project description:Genome-wide maps of Runx3 bound regions in IL-2-activated splenic NK cells