Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane, canola and diesel as carbon source.
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane, canola and diesel as carbon source. Two-condition experiment, cells grown on pyruvate vs. cells grown on hexadecane, canola or diesel. For each condition 4 replicates were used, as for the control condition also 4 replicates were used for analysis.
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane as carbon source, that were stressed with 1- octanol. The gene expression was measured 15 min, 30 min, 60 min and 90 min after 1-octanol addition.
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane as carbon source, that were stressed with 1- octanol. The gene expression was measured 15 min, 30 min, 60 min and 90 min after 1-octanol addition. Two-condition experiment, 1-octanol-stressed vs. unstressed cells; different time points were investigated after 1-octanol was spiked in (15 min, 30 min, 60 min, 90 min); for each condition 3 replicates were used, for the control condition 4 replicates (pyruvate cultures) and 3 replicates (hexadecane cultures) were used for analysis
Project description:The marine bacterium Rhodococcus erythropolis PR4 was demonstrated to be able for assimilation/biodegradation of hydrocarbons. Not just the chromosome but two large plasmids provide versatile enzyme sets involved in many metabolic pathways. In order to identify the key elements involved in biodegradation of the model compound, hexadecane, and diesel oil, we performed whole transcriptome analysis on cells grown in the presence of n-hexadecane and diesel oil. Sodium acetate grown cells were used as control. The final goal of the project is a comparative transcriptomic analysis of Rhodococcus erythropolis PR4 cells grown on acetate, on the model compound: hexadecane and the real substrate: diesel oil.
Project description:The marine bacterium Rhodococcus erythropolis PR4 was demonstrated to be able for assimilation/biodegradation of hydrocarbons. Not just the chromosome but two large plasmids provide versatile enzyme sets involved in many metabolic pathways. In order to identify the key elements involved in biodegradation of the model compound, hexadecane, and diesel oil, we performed whole transcriptome analysis on cells grown in the presence of n-hexadecane and diesel oil. Sodium acetate grown cells were used as control. The final goal of the project is a comparative transcriptomic analysis of Rhodococcus erythropolis PR4 cells grown on acetate, on the model compound: hexadecane and the real substrate: diesel oil. Comparative transcriptomics of Rhodococcus erythropolis PR4 grown on n-hexadecane, diesel oil, and sodium acetate.
