Project description:The marine obligate hydrocarbonoclastic bacterium Thalassolituus oleivorans MIL-1 metabolises a broad range of aliphatic hydrocarbons almost exclusively as carbon and energy sources. We used LC-MS/MS shotgun proteomics to identify proteins involved in aerobic alkane degradation during growth on medium- (n-C14) or long-chain (n-C28) alkanes.
Project description:Transcriptional profiling of A. oleivorans DR1 cells comparing control untreated wild type cells with norfloxacin treated wild type cells. To identify genes important for norfloxacin stress response in A. oleivorans DR1, the cells were grown to exponential phase (OD600 ~0.5) and treated with norfloxacin (4?g/ml) over a period of 15 min.
Project description:An exponentially growing culture of strain R6 in AGCH at OD620nm=0.4 was either non-treated or treated with two LVX concentrations: 0.125 ug/ml LVX (0.5x MIC of R6) and 2.5 ug/ml LVX (10x MIC of R6). Samples were taken before treatment (0 min), at 15, 30 and 60 min in the non-treated culture, and at 5, 15 ,30 and 60 min in the LVX treated cultures
