Project description:Physcomitrium (Physcomitrella) patens Gransden wild type strain and four mutants Δdek1 (Perroud et al., 2014); Δloop (Demko et al., 2014); Δlg3 (Johansen et al., 2016) and oex1 (this work) were sequenced for time series analysis.
Project description:To investigate the effects of dcl3,dcl4,mDCL,rdr2,rdr6,nrpd1b, and nrpd1c mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed.
Project description:To investigate the effects of rdr2 and rdr6 mutations upon the strucutred component of the Physcomitrella patens transcriptome, dsRNAseq performed. The accumulation of dsRNAseq reads within known small RNA (microRNA and siRNA) loci was quantified and compared between wild-type and the two mutants.
