Project description:PU.1 is an Ets family transcription factor that is essential for the differentiation of both myeloid and lymphoid cells. PU.1 is down-regulated in classical Hodgkin lymphoma cells via methylation of the PU.1 promoter. To evaluate whether down-regulation of PU.1 is essential for the growth of cHL cells, we generated KM-H2 derived cell lines conditionally express PU.1 by tet-off system (designated KM-H2tetPU.1). Conditonally expressed PU.1 by tetracycline removal induced complete growth arrest and apoptosis in KM-H2 cells. To elucidate the mechanisms underlying cell cycle arrest and apoptosis induced by PU.1, we compared gene expression profiles of KM-H2tetPU.1 cells 0, 1 and 3 days after PU.1 induction, by DNA microarray.

2012-11-22 | GSE42440 | GEO

Transcriptome analysis of KM-H2 cells with different Rhodamin 123 eflux capacity

Project description:Rhodamin 123 is a dye which can be used to detect the activity of ABC transporters. We observed that after staining of KM-H2 Hodgkin lymphoma cells with Rhodamin 123, part of the cells rapidly eliminated the dye, while another part of the cells retained the dye for a longer time. We compared the transcriptome of KM-H2 Hodgkin lymphoma cells with high Rhodamin 123 efflux capacity and KM-H2 cells with low Rhodamin 123 efflux capacity.

2021-01-06 | GSE164318 | GEO

ChIP-seq from midbrain (ENCSR601HSS)

Project description:Chip-Seq on P0 brain, midbrain-mouse (fragmentation date:Thu Feb 05 2015 00:00:00 GMT-0600 (CST) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE91905 | GEO

Expression changes induced by PTPN1 knockdown in KM-H2 cells

Project description:Gene expression data for shRNA PTPN1 knockdown vs. Non-silencing in the classical Hodgkin lymphoma-derived cell line KM-H2

2014-01-17 | GSE54157 | GEO