Project description:Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at variable light and temperature conditions under greenhouse environment (period March-June). Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation. Seeds of Medicago truncatula were collected at different developmental stages, 9 developmental stages were analysed. Two replicates from each developmental stage were used for dye switch, each time the control was considered as the earlier developmental stage vs the treatment corresponding to the later developmental stage. For each repetition 5 hybridisation were made: 16DAP vs 20DAP, 24DAP vs 28DAP, 32DAP vs 36DAP, 40DAP vs Abs, DS (Rep1 and Rep2) vs 16DAP (Rep3 and Rep4). For each biological replicates, RNA was extracted from 50 seeds collected from 5 different plants, grown in the same conditions. One replicate per array.

2013-12-05 | E-GEOD-52833 | biostudies-arrayexpress

Medicago truncatula seed development at 21-19°C

2013-07-31 | GSE49350 | GEO

Medicago truncatula seed development under greenhouse conditions

Project description:Medicago truncatula seed development under greenhouse conditions

| PRJNA564745 | ENA

Medicago truncatula seed development at 14/11°C 16 light/dark

2013-12-18 | GSE53002 | GEO

Medicago truncatula seed development at 20/18°C 16 h light/dark in osmotic stress conditions: -0.1 Mpa

2013-12-05 | GSE53003 | GEO

Transcriptome of Medicago truncatula abi5 mutants during seed development

Project description:A transcriptome analysis of developing abi5 and wild type (R108) seeds from Medicago truncatula was performed to decipher the role of ABI54 in the regulation of late seed maturation and seed longevity.

2016-09-24 | GSE87313 | GEO

Medicago truncatula seed development at 26/24°C 16 h light/dark

2013-12-05 | GSE52832 | GEO

Medicago truncatula late seed maturation

Project description:Seven developmental stages of Medicago truncatula A17 during seed maturation (from 24 DAP to dry seeds)

2013-07-23 | E-MEXP-3719 | biostudies-arrayexpress