Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani. Agilent one-color experiment,Organism: Leishmania donovani, Microarray GeneExpression 8x15k designed by Genotypic Technology Private Limited. (AMADID: 035638)
Project description:Differential gene expression analysis was carried out in clinical isolates of Leishmania donovani (L. donovani) parasite derived from VL patient before miltefosine treatment, post treatment and patient that relpased after miltefosine treatment
Project description:Gene expression analysis was carried out between experimental miltefosine resistant L.donovani (adapted to 30ug/ml miltefosine drug pressure) and its corresponding wild type miltefosine senstive parasite 3 biological replicates of lab generated miltefosine resistant Leishmania donovani (K417 M30) were compared with corresponding wild type sensitive parasite (K417WT)
Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani.
Project description:Monastrol treatment of Leishmania donovani infected macrophages Macrophages were infected with Leishmania donovani and treated with monastrol to look for signalling molecules
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani.
Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.
