Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.
Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.
Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. Fresh Livers from 40 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used.
Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. Fresh Livers from 40 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used.
Project description:In this study, Solexa deep sequencing technology was used for high-throughput analysis of miRNAs in a small RNA library isolated from serum sample of HCV-related fibrosis and control healthy. In total, 41 miRNAs were dysregulated (30 upregulated and 11 downregulated) in the patients with chronic HCV infection compared with the healthy controls. Furthermore, miRNA features including length distribution and end variations were characterized. Annotation of targets revealed a broad range of biological processes and signal transduction pathways regulated by HCV-induced fibrosis miRNAs. In addition, miRNAs of HCV-related fibrosis and control healthy were confirmed using miRNA microarray analysis. Real-time quantitative PCR (qPCR) analysis of miRNA in the chronic HCV infection patients and control healthy groups showed good concordance between the sequencing and qPCR data. This study provides the first large-scale identification and characterization of HCV-related fibrosis miRNAs and their potential targets, and represents a foundation for further characterization of their roles in the regulation of the diversity of HCV-related fibrosis.
