Project description:We sequenced mRNA in grossly enlarged testes from 1-year-old Stra8-deficient mice, and in testes from adult male wild-type controls, to verify that Stra8-deficient testes are enriched for genes normally expressed in type A spermatogonia.
Project description:Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform.
Project description:Microarray analysis of wild type plants and plants with reduced (ago1-27 and se-1) or increased miR156 levels (se-1 p35S:MIR156). Shoot apices were dissected from 20-day-old, short-day grown plants.
Project description:Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform. The following mice were analyzed at postnatal day 7: wild-type, Sohlh1-/-, and Sphlh2-/-. 4 samples/group.
Project description:We sequenced mRNA in grossly enlarged testes from 1-year-old Stra8-deficient mice, and in testes from adult male wild-type controls, to verify that Stra8-deficient testes are enriched for genes normally expressed in type A spermatogonia. Examination of mRNA levels in 6 whole-testis samples (3 replicates of each genotype). Specifically, we sequenced mRNA in grossly enlarged testes from three 1-year-old Stra8-deficient male mice, and in normal testes from three adult male wild-type controls
Project description:To identify RFX2-dependent genes during spermatogenesis, We chose 24-day-old wild-type and mutant testes samples. Spermiogenesis in mutants was normal before this time point while massive round spermatids detached from the tubules thereafter. RNAs were isolated from the total testicular cells.
Project description:To identify a physiologic postanal transcriptomic program between postnatal day 20 and 60, we first analyzed the gene expression profile in 60 day-old ( young adult) wild-type mice (WT) and compared it to 20 day-old WT mice To identify the gene expression between postnatal day 20 and 60 under the strict dependence of cardiac ephrin-B1, we compared gene expression in 20 day-old and 60 day-old Efnb1 CMspe KO (a-MHC-Cre-/+ Efnb1 flox/flox or) to 20 day-old and 60 day-old WT mice
Project description:To futher find the gene expression due to the aromatase overexpression in the mouse testis,we have employ whole genome microarray expression profiling as a discovery platform to identify genes compared to the wild type mice. The testes were taken from 5 month old aromatase overexpression mice and WT mice as control.
