Bos taurus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA243569 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Successful establishment and maintenance of pregnancy can be attained only through optimum conceptus-maternal cross talk. Despite significant progress in our understanding of the temporal changes in the transcriptome of the uterine endometrium, we have only a rudimentary knowledge of the genes and pathways governing growth and development of the bovine conceptus. In particular, very little information exists for the posthatchingembryo and elongating conceptus. This period of development is arguably the most important, as approximately 40% of all embryonic loss occurs between Days 8 and 17 of pregnancy in cattle. Here, we describe the global transcriptome profile of the bovine conceptus at five key stages of its pre- and peri-implantation growth (Days 7, 10, 13, 16, and 19) using state-of-the-art RNA sequencing techniques. More than 287 million reads were generated at the five stages, and more than 22?700 unique transcripts were detected. Analysis of variance followed by self-organizing maps identified differentially regulated (P < 0.05) genes organized in nine gene clusters forming a sequential transcript dynamics across these developmental stages. Of particular interest, genes in clusters 3 (n = 236) and 6 (n = 1409) were significantly up-regulated on Days 16 and 19, suggesting a role in maternal recognition and initiation of implantation. This transcriptome analysis of the bovine conceptus will provide a blueprint of the dynamic changes in gene expression occurring during maternal recognition and implantation and will complement existing knowledge of the temporal changes in the endometrial transcriptome, thus facilitating a better understanding of conceptus-maternal cross talk during the peri-implantation period of pregnancy. mRNA-seq study of bovine conceptuses at 5 stages of development (days 7, 10, 13, 16 and 19) post fertilization.
2014-04-04 | E-GEOD-56513 | biostudies-arrayexpress
Project description:Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of embryo development and implantation in mammals via posttranscriptional gene regulation mechanisms. Applying custom made multispecies arrays we aimed to analyze expression profile of microRNAs in peri-implantation porcine conceptuses/trophoblasts to identify their potential role at the maternal-fetal interface during the critical period of maternal recognition of pregnancy and implantation. miRNA expression profiles were analyzed in samples collected from embryos or trophoblast on Days 10, 11, 12, 16 and 20 of pregnancy. Each group was represented by five to nine samples.
2014-12-05 | E-GEOD-59618 | biostudies-arrayexpress
Project description:Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of embryo development and implantation in mammals via posttranscriptional gene regulation mechanisms. Applying custom made multispecies arrays we aimed to analyze expression profile of microRNAs in peri-implantation porcine conceptuses/trophoblasts to identify their potential role at the maternal-fetal interface during the critical period of maternal recognition of pregnancy and implantation.
2014-12-05 | GSE59618 | GEO
Project description:Successful establishment and maintenance of pregnancy can be attained only through optimum conceptus-maternal cross talk. Despite significant progress in our understanding of the temporal changes in the transcriptome of the uterine endometrium, we have only a rudimentary knowledge of the genes and pathways governing growth and development of the bovine conceptus. In particular, very little information exists for the posthatchingembryo and elongating conceptus. This period of development is arguably the most important, as approximately 40% of all embryonic loss occurs between Days 8 and 17 of pregnancy in cattle. Here, we describe the global transcriptome profile of the bovine conceptus at five key stages of its pre- and peri-implantation growth (Days 7, 10, 13, 16, and 19) using state-of-the-art RNA sequencing techniques. More than 287 million reads were generated at the five stages, and more than 22 700 unique transcripts were detected. Analysis of variance followed by self-organizing maps identified differentially regulated (P < 0.05) genes organized in nine gene clusters forming a sequential transcript dynamics across these developmental stages. Of particular interest, genes in clusters 3 (n = 236) and 6 (n = 1409) were significantly up-regulated on Days 16 and 19, suggesting a role in maternal recognition and initiation of implantation. This transcriptome analysis of the bovine conceptus will provide a blueprint of the dynamic changes in gene expression occurring during maternal recognition and implantation and will complement existing knowledge of the temporal changes in the endometrial transcriptome, thus facilitating a better understanding of conceptus-maternal cross talk during the peri-implantation period of pregnancy.
2014-04-04 | GSE56513 | GEO
Project description:The majority of pregnancy loss in cattle occurs between days 8 and 16 of gestation coincident with the initiation of conceptus elongation and the onset of maternal recognition of pregnancy. Differences in conceptus lengths on the same day of gestation may be related to an inherent lack of developmental competency or may simply be a consequence of asynchrony with the maternal environment. The primary objective of this work was to characterize differential patterns of mRNA expression between short and long bovine conceptuses recovered on Day 15 of gestation. Embryo selection is an important factor that contributes to pregnancy success following transfer of embryos produced in vivo or in vitro. Morphological evaluation of embryos for stage of development and quality grade is the most widely used method for predicting embryo viability in cattle. Multiple studies have demonstrated that there are significant differences in pregnancy rates across different morphological grades. However, morphological evaluation is not always the best predictor of pregnancy success. Therefore, it is of interest to develop an objective and highly predictive method of pre-transfer embryo screening. A secondary objective of this experiment was to characterize differential patterns of mRNA expression between Day 15 bovine conceptuses derived from Grade 1 (excellent) and Grade 3 (poor) embryos. In Experiment 1, gene expression was compared between Day 15 conceptuses derived from the transfer of Grade 1 (n=4) versus Grade 3 (n=3) embryos. In Experiment 2, gene expression was compared between Grade 1-derived Day 15 conceptuses classified as either short (n=5) or long (n=5) in length.
2015-12-08 | E-GEOD-75750 | biostudies-arrayexpress
Project description:The majority of pregnancy loss in cattle occurs between days 8 and 16 of gestation coincident with the initiation of conceptus elongation and the onset of maternal recognition of pregnancy. Differences in conceptus lengths on the same day of gestation may be related to an inherent lack of developmental competency or may simply be a consequence of asynchrony with the maternal environment. The primary objective of this work was to characterize differential patterns of mRNA expression between short and long bovine conceptuses recovered on Day 15 of gestation. Embryo selection is an important factor that contributes to pregnancy success following transfer of embryos produced in vivo or in vitro. Morphological evaluation of embryos for stage of development and quality grade is the most widely used method for predicting embryo viability in cattle. Multiple studies have demonstrated that there are significant differences in pregnancy rates across different morphological grades. However, morphological evaluation is not always the best predictor of pregnancy success. Therefore, it is of interest to develop an objective and highly predictive method of pre-transfer embryo screening. A secondary objective of this experiment was to characterize differential patterns of mRNA expression between Day 15 bovine conceptuses derived from Grade 1 (excellent) and Grade 3 (poor) embryos.
2015-12-08 | GSE75750 | GEO
Project description:Sexually dimorphic gene expression in bovine conceptuses at the initiation of implantation is not reflected in the changes in the endometrial transcriptome.
| PRJNA305273 | ENA
Project description:In cattle, maternal recognition of pregnancy occurs on Day 16 via secretion of interferon tau (IFNT) by the conceptus. The endometrium can distinguish between embryos with different developmental competencies. In eutherian mammals, X-chromosome inactivation (XCI) is required to ensure an equal transcriptional level of most X-linked genes for both male and female embryos in adult tissues, but this process is markedly different in cattle than mice. We examined how sexual dimorphism affected conceptus gene expression and amino acid composition as well as the endometrial transcriptome during the peri-implantation period of pregnancy. Of the 5132 genes were differently expressed on Day 19 in male compared to female conceptuses, 2.7% were located on the X-chromosome. Concentrations of specific amino acids were higher in the uterine luminal fluid with male compared to female conceptuses, while female conceptuses had higher expression of specific amino acid transporters (SLC6A19 and SLC1A35). Of note, the endometrial transcriptome was not different in cattle gestating a male or a female conceptus. These data support the hypothesis that, far from being a blastocyst specific phenomenon, XCI is incomplete before and during implantation in cattle. Despite differences in gene expression and amino acid utilization in male versus female conceptuses, the sex of the conceptus itself does not elicit a different response in the endometrium.
2016-03-01 | GSE75754 | GEO
Project description:Transcriptional profiles of bovine in vivo pre-implantation development
| PRJNA254699 | ENA