Project description:Transient transfection of TARBP2 expression vectors (along with GFP controls) followed by transcriptomic profiling revealed that over-expression of TARBP2 resulted in a significant enrichment of sRSE-carrying transcripts among the down-regulated transcripts. Transient transfection of CMV-driven TARBP2 was used to over-express this protein in MDA-231 cells. The cells were then subjected to transcripome profiling in comparison to mock-transfected cells.
Project description:To characterize the effect of Core regulatory element (CRE) deletion in breast cancer cell line (MDA-MB-231 cells), we performed gene expression RNA-seq analysis for WT and KO (Core Regulatory Element deleted) MDA-MB-231 cells after 0h, 6h and 24h of P/I treatment.