Project description:Analysis of U251 glioblastoma multiforme (GBM) cells treated with a new culcita novaeguineae asterosaponion, CN-3. A new asterosaponin was isolated from culcita novaeguineae, an abundant marine resource in the south China sea. The asterosaponin induced significant growth inhibition with a 50% inhibitory concentration at 48 h of 2.013 μg/mL in U251MG cells. 1.8μg/mL of the asterosaponin reduced U251 MG cells viability from 100 % to 42.5% (24 h), 37.4% (48 h) and 52.1% (72 h). In this study, a microarray analysis was performed using RNA prepared from U251MG GBM cells treated with the asterosaponion. These data revealed that 661 genes had significant differential expressions.
Project description:Viral concentrates (VCs) contained both viral particles and cell-affiliated components. In this study, a shotgun metaproteomic approach was applied to characterize proteins in the VCs collected from chlorophyll maximum, mesopelagic (200 m) and bathypelagic (3000 m) waters in the South China Sea. Abundant viral proteins indicated the shift of viral community as depth. Whereas the remaining non-viral proteins suggested the diverse microbial metabolism distinct at different layers.
