Project description:The aim of this project was to detect S. cerevisiae fragments in the genome of S. bayanus Type strain CBS380 and pure line NBRC1948 (=IFO1948). The hybridization signal is compared to S. uvarum BBS7001 whose genome has been sequenced and in which no S. cerevisiae sequence has been detected. Comparison CBS380 (2 arrays) or IFO1948 (one array) to CBS 7001 (2 arrays).
Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Saccharomyces bayanus genes Total RNA was collected in mid-log phase from Saccharomyces bayanus cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Saccharomyces bayanus.