Project description:Purpose: The goal of this study is to compare NGS-derived transcriptome profiling (RNA-seq) in AD patients’ hippocampus. Methods: mRNA profiles of human hippocampus from AD patients and healthy controls were generated by deep sequencing, using Illumina GAIIx. Results: Using an optimized data analysis workflow, more than 20000 transcripts in hippocampus were detected. Top 6 genes were selected and related to “ion transport”. Correlation analysis demonstrated negative relationship of gene expression level with the neuropathologic changes.
Project description:To understand the genetic regulation of gene expression and patterns of gene co-expression, we sequenced the transcriptome of the hippocampus of 258 Diversity Outbred (DO) mice of both sexes. DO mice (fourth and fifth generations of outcrossing) were sacrificed between 6-8 weeks of age and hippocampus dissected. Total hippocampal RNA was isolated using a TRIzol Plus RNA purification kit (Life Technologies) and mRNA sequencing library was prepared using a TruSeq kit (Illumina), both according to manufacturer's protocols. Paired-end 100bp reads were obtained using the Illumina HiSeq 2000.
Project description:Little is understood about the underlying cellular and molecular mechanisms related to hippocampus damage and glial activation after severe cerebral hypoperfusion.Efforts to explore the relationship between neuropathological and gene expression changes support a role for identifying novel molecular pathways by transcriptomic mechanisms. To investigate hippocampus-specific transcriptome profiling after severe cerebral hypoperfusion in mice
