Project description:We report the genome-wide small RNA of soybean early maturation seed coat parenchyma compartment soybean early maturation seeds using Illumina high-throughput sequencing technology. Illumina sequencing of small RNA from early maturation seed coat parenchyma compartment and early-maturation stage whole seeds
Project description:A transcriptome analysis of soybean seeds harvested at different developing stages (between stage 7.1 and stage 9) was carried out to understand the molecular events occuring during the acquisition of seed longevity during maturation.
Project description:RNA-seq was used to characterize gene expression in soybean from a wide range of tissues. The primary focus of the project was small RNAs, and the identification of microRNAs and phased siRNA-generating loci, but RNA-seq data were generated from the same samples. This project was supported by the United Soybean Board.
Project description:We microdissected each compartment from 8-micron paraffin sections using the Leica AS LMD system to identify all genes active in different compartments of a soybean seed containing a heart-stage embryo. Experiment Overall Design: heart-stage seed compartments were isolated using the Leica AS LMD system. Total RNA was amplified and hybridized with Affymetrix Soybean Genome Arrays.