Project description:1, Using mRNA-Seq to get expression profiling of asi1-1 mutant and WT; 2, Using MethylC-Seq to provide single-base resulution of DNA methylation status in asi1-1 mutant mRNA-Seq: 2 samples examined, Col-0 wild-type with 35S-SUC2 transgene(termed as WT) and asi1-1 mutant (Col-0 background with 35S-SUC2 transgene); MethylC-Seq: 1 sample examined, asi1-1 mutant
Project description:Using BS-Seq to provide single-base resolution of DNA methylation status in 35S-SUC2 WT and antisilencing mutants (arp6, pie1, h2a.z, idm1, and ros1)
Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in ros1-13 mutant Whole genome methylation maps of ros1-13 (with 35S-SUC2 transgene) was generated using BS-seq
Project description:We examined global expression profiles of 7-days old 35S-TrAP transgenic plants compared to Col-0 wild-type using an Affymetrix ATH1 GeneChip and identified 586 genes that are differentially expressed in the 35S-TrAP transgenic plants (q<0.005). Of these, 295 transcripts were elevated whereas 291 were reduced (Figure 2E). We performed real-time PCR and RNA blot assays to validate the microarray results for the differentially expressed genes (DEGs).
Project description:The Arabidopsis thaliana Myb transcription factor, FE, acts as a key regulator of phase transition. In order to identify potential target genes of FE protein, we performed microarray experiments. Using fe-1 and transgenic plants overexpressing GR-tagged FE (35S::FE-GR), we compared transcriptional profiling of WT (L.er) vs fe-1 and Dex-treated 35S::FE-GR vs Mock-treated 35S::FE-GR. Transcriptional profiling of A. thaliana comparing WT (L.er) with the fe-1 mutant
Project description:We performed immunoprecipitation analysis with leaves from 35S::NusG:MYC transgenic lines throung the MYCantibody, and the product was subjected to LC-MS analysis.
