Project description:In the current study we have profiled changes in the transcriptome of somatic and reproductive tissues of B.rapa plants following heat shock stress exposure. For this we used Illumina GAIIx platform. We have demonstrated that the highest tissue-specific alterations in the transcriptome profile are detected in tissues that were not directly exposed to stress, namely, in the endosperm and pollen. The progeny of exposed plants also exhibited significant alterations in the gene expression as compared to progeny of control plants. Overall design: The transcriptome profile was assessed after heat shock treatment in the following tissues of Brassica rapa plants: parental leaves, inflorescence meristem, pollen, unfertilized ovules, 24-hour post-fertilization ovules, embryo, endosperm and leaf tissues of progeny plants. Please note that *diff_out.tar.gz contains all the standard output produced by cuffmerge and cuffdiff and each file content/format details are provided in the 'README.txt' file
Project description:We report here NGS RNA-seqencing datasets for two different light condition of two different Brassica rapa cultivars, vegetable-type "Chiifu" and oilseed-type "LP08" plants
Project description:We report here NGS RNA-seqencing datasets for tive different vernalization time course of two different Brassica rapa cultivars, vegetable-type "Chiifu" and oilseed-type "LP08" plants
Project description:Among Brassica rapa, rapid cycling Brassica rapa and Brassica rapa inbred line Kenshin showed contrasting leaf morphology. To identify genes associated with leaf morphology, four distinct F2 progeny of RcBr X Kenshin cross and their parents were selected. Leaf samples were collected from 6 materials, isolated total RNA, and subjected to newly developved 135K microarray. Experiments were performed with three or two biologic
Project description:Transcription profiling of Brassica rapa, Brassica oleracea and Brassica napus I and II The nuclear genomes of the resynthesised B. napus lines should be identical but, as one (B. napus I) involved a cross of B. oleracea onto B. rapa, and the other (B. napus II) involved a cross of B rapa onto B. oleracea, they differ in cytoplasm, and hence contain different chloroplast and mitochondrial genomes.
Project description:Root and leaf samples from Brassica rapa line R-O-18 were compared. The results will be compared to the same samples hybridised to the Affymetrix Brassica Exon 1.0 ST array. 6 samples were hybridised. Triplcate samples of 11 day old roots and 2 semi-expanded leaves from 23 day old Brassica rapoa R-O-18 plants.
Project description:The aim of the experiment was to evaluate the performance of the Affymetrix Brassica Exon 1.0 ST array. Root and leaf samples from Brassica rapa line R-O-18 were compared. The same RNA samples were hybridised to the Agilent Brassica array, to compare the performance of the two arrays. 6 samples were hybridised to each array. Triplicate samples of 11-day-old roots and 2 semi-expanded leaves from 23-day-old Brassica rapoa R-O-18 plants.