Genomics

Dataset Information

118

Agrobacterium tumefaciens


ABSTRACT: Identification of Hfq bound RNA by co-immunoprecipitation and RNA-sequencing

ORGANISM(S): Agrobacterium tumefaciens  

PROVIDER: PRJNA254473 | ENA |

REPOSITORIES: ENA

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Publications

Profound impact of Hfq on nutrient acquisition, metabolism and motility in the plant pathogen Agrobacterium tumefaciens.

Möller Philip P   Overlöper Aaron A   Förstner Konrad U KU   Wen Tuan-Nan TN   Sharma Cynthia M CM   Lai Erh-Min EM   Narberhaus Franz F  

PloS one 20141017 10


As matchmaker between mRNA and sRNA interactions, the RNA chaperone Hfq plays a key role in riboregulation of many bacteria. Often, the global influence of Hfq on the transcriptome is reflected by substantially altered proteomes and pleiotropic phenotypes in hfq mutants. Using quantitative proteomics and co-immunoprecipitation combined with RNA-sequencing (RIP-seq) of Hfq-bound RNAs, we demonstrate the pervasive role of Hfq in nutrient acquisition, metabolism and motility of the plant pathogen A  ...[more]

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