Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture for 7 days Human MSC line (hMSC-TERT) were subjectd to adipocytic differentiation for 7 days, subsequently, RNA was extracted using Norgen total RNA isolation kit. Extracted RNA was labeled and then hybridized to the one-color Agilent Human GE 8x60K Microarray chip.
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture for 7 days
Project description:We have employed whole microRNA microarray to identify changes in microRNA expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture on day 7 and day 13
Project description:We performed small RNA sequencing on telomerized human bone marrow skeletal (stromal or mesenchymal) stem cells (hMSC-TERT) at various timepoints during differentiated into osteoblasts
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) upon differentiation into adipocytes (day 7)
Project description:We have employed whole microRNA microarray to identify changes in microRNA expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture on day 7 and day 13 Human MSC line (hMSC-TERT) were subjectd to adipocytic differentiation for 7 days or 13 days, subsequently, RNA was extracted. The samples were labeled using the miRCURY LNAM-bM-^DM-" microRNA Hi-Power Labeling Kit, Hy3M-bM-^DM-"/Hy5M-bM-^DM-" and hybridized on the miRCURY LNAM-bM-^DM-" microRNA Array (6'th GEN). Array product number 208400; Array version 6'th GEN, Array batch number 34014, miRBase version 17. The 'Legends_rawdata.pdf' contains the column headers (of raw data files) and their detailed description.
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) transfected with miR-320c or miR-negative control at 72hrs post-transfection
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) sorted from seven days co-cultures with breast cancer cell lines (MCF7) and (BT-20)
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) transfected with miR-320c or miR-negative control at 72hrs post-transfection Human MSC line (hMSC-TERT) were transfected with miR-320c (30 nM) or miR-negative control (30 nM), subsequently, RNA was extracted using Norgen total RNA isolation kit at 72hrs post-transfection. Extracted RNA was labeled and then hybridized to the one-color Agilent Human GE 8x60K Microarray chip.
Project description:We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) sorted from seven days co-cultures with breast cancer cell lines (MCF7) and (BT-20) Human MSC line (hMSC-TERT) were co-cultured with breast cancer cell lines MCF7 and BT-20 for seven days then were sorted using EpiCAM MACS beads. Subsequently, RNA was extracted using Norgen total RNA isolation kit. Extracted RNA was labeled and then hybridized to the one-color Agilent Human GE 8x60K Microarray chip.
