Global ChIP-seq Normalization Reveals Epigenome Modulations
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ABSTRACT:
PROVIDER: PRJNA257491 | ENA |
REPOSITORIES: ENA
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2014-10-30 | E-GEOD-60104 | biostudies-arrayexpress
Project description:Epigenomic profiling by ChIP-seq is a prevailing methodology used to investigate chromatin-based regulation in biological systems, such as human disease, yet the lack of an empirical methodology to normalize amongst experiments has limited the usefulness of this technique. Here we describe a “spike-in” normalization method that allows the quantitative comparison of histone modification status across cell populations using defined quantities of a reference epigenome. We demonstrate the utility of this method in measuring epigenomic changes following chemical perturbations and show how control normalization of ChIP-seq experiments enables discovery of disease-relevant changes in histone modification occupancy.
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