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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

ABSTRACT: DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

PROVIDER: PRJNA259204 | ENA |

REPOSITORIES: ENA

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Project description:DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

Project description:This SuperSeries is composed of the SubSeries listed below.

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [MeDIP]

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

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Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state of human general transcription factor TFIID

Project description:General transcription factor TFIID is a key component of RNA polymerase II transcription initiation in eukaryotic nuclei. Human TFIID is a megadalton-sized multiprotein complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. A number of transcription regulatory factors were found to compete with DNA for TBP binding. We identified a ternary complex formed by TBP and the histone fold (HF) domain containing TFIID subunits TAF11 and TAF13. We demonstrate that TAF11/TAF13 competes for TBP binding with TATA-box DNA, and also with the N-terminal domain of TAF1. In an integrative approach combing crystal coordinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the architecture of the TAF11/TAF13/TBP complex, revealing TAF11/TAF13 interaction with the DNA binding surface of TBP. Our results thus suggest a novel regulatory state for TFIID function.

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