Transcriptome data of interacting Salmonella Typhimurium and human host cells
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ABSTRACT:
PROVIDER: PRJNA261966 | ENA |
REPOSITORIES: ENA
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Project description:The poly(A)+ and poly(A)− fractions of interacting and non-interacting cells were used for distinct library preparation of interacting and non-interacting prokaryotic pathogen and eukaryotic host cells by deepSuperSAGE. Sequencing was performed with the Illumina HiSeq 2000 platform, and one point of time post infection (early interaction) was additionally prepared by Massive Analysis of cDNA Ends (MACE) as alternative tag-based library preparation method.
2015-05-12 | GSE61730 | GEO
Project description:The poly(A)+ and poly(A)− fractions of interacting and non-interacting cells were used for distinct library preparation of interacting and non-interacting prokaryotic pathogen and eukaryotic host cells by deepSuperSAGE. Sequencing was performed with the Illumina HiSeq 2000 platform, and one point of time post infection (early interaction) was additionally prepared by Massive Analysis of cDNA Ends (MACE) as alternative tag-based library preparation method. 10 deepSuperSAGE and 2 MACE libraries. Please consult the publication mentioned in the following for more details.
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2009-03-02 | GSE13994 | GEO
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Project description:During an infection, bacteria are exposed to the complex host immune response and has to adapt to the changing environment in order to successfully grow within tissues. In the present sturdy we analysed the transcriptome of both host (mouse) and bacteria (Salmonella Typhimurium) at different time points during the infection to gain a better insight into the attack mounted by the host to repel the bacterial infection and how the bacteria adapts to it. For this, mice were infected intravenously with Salmonella Typhimurium and spleens were collected for RNA isolation at specific time points. Transcription profile of infected mice was compared to uninfected controls.
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