Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA263477 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:DNA methylation profiling in Wild Type (WT), mutant (ddm1) and epiRILs (060-098-202-260-344-480)
2014-10-10 | GSE62206 | GEO
Project description:DNA methylation profiling in Wild Type (WT), mutant (ddm1) and epiRILs (060-098-202-260-344-480) 1 biological replicate per sample - Bisulfite-seq
2014-10-10 | E-GEOD-62206 | biostudies-arrayexpress
Project description:Methylome analysis of 123 epiRILs, WT and ddm1 mutant aerial part
| PRJNA159401 | ENA
Project description:mRNA levels in Wild-type versus ddm1 Arabidopsis thaliana seedlings and bolting plants. Features found to be significantly enriched for DNA methylation were determined. This SuperSeries is composed of the following subset Series: GSE1324: EV23+24 mRNA levels in Wild-type versus ddm1/+ backcross bolting Arabidopsis thaliana plants GSE1325: EV33+34 mRNA levels in Wild-type versus ddm1 Arabidopsis thaliana seedlings GSE1326: VC109+111 mRNA levels in Wild-type versus ddm1 Arabidopsis thaliana seedlings GSE1327: EV39+40 mRNA levels in Wild-type versus ddm1 Arabidopsis thaliana seedlings GSE1328: VC110+112 mRNA levels in Wild-type versus ddm1 bolting Arabidopsis thaliana plants Refer to individual Series
2010-06-09 | E-GEOD-1354 | biostudies-arrayexpress
Project description:Histone 3 lysine 4 and histone 3 lysine 9 methylation in wild type and ddm1 Arabidopsis thaliana seedlings. The purpose of the chromatin immunoprecipitation/microarray (ChIP/chip) experiment is to determine which regions of a genome are enriched for a particular histone modification in a single Arabidopsis thanliana genotype. Chromatin immunoprecipitation with antibodies raised against dimethyl histone-H3 lysine-9 (H3mK9) or dimethyl histone-H3 lysine-4 (H3mK4) is performed on a selected genotype. This purified DNA from each immunoprecipiation (mH3K9, mH3K4, no antibody control) is used for random amplification to increase the quantity of DNA for microarray hybridization. The amplified DNA from each experimental sample is then labeled with Cy5 and hybridized against total input DNA from the corresponding genotype, labeled in Cy3. In a single hybridization, the total input DNA serves as a baseline and is compared to the immunoprecipitated samples. Ratios of normalized signal intensities were calculated to identify enrichment of a particular sequence after immunoprecipitation, in comparison to the total input DNA. Dye swap analysis is carried out to take account of experimental variation by repeating the hybridization with identical samples labeled with Cy3 and Cy5, respectively. This SuperSeries is composed of the following subset Series: GSE1333: EV49+50, Histone 3 Lysine 4 methylation in wild-type Arabidopsis thaliana seedlings GSE1334: Histone 3 Lysine 4 methylation in ddm1 Arabidopsis thaliana seedlings GSE1335: EV104+105, Histone 3 Lysine 4 methylation in ddm1 Arabidopsis thaliana seedlings GSE1336: Ev106+107, Histone 3 Lysine 4 methylation in WT Arabidopsis thaliana seedlings GSE1337: EV51+52, Histone 3 Lysine 9 methylation in wild-type Arabidopsis thaliana seedlings GSE1338: EV59+60, Histone 3 Lysine 9 methylation in ddm1 Arabidopsis thaliana seedlings GSE1339: Histone 3 Lysine 9 methylation in wild-type Arabidopsis thaliana seedlings GSE1340: EV110+111, Histone 3 Lysine 9 methylation in ddm1 Arabidopsis thaliana seedlings Refer to individual Series
2010-06-09 | E-GEOD-1356 | biostudies-arrayexpress
Project description:Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. In this study we aimed to understand and quantify the contribution of DNA methylation variation to heterosis in various traits of Arabidopsis. Therefore we created epigenetic hybrid lines from ddm1-derived epiRILs. We performed whole genome sequencing on four epiHybrids and their parental lines (epiRILs & Col-wt) in duplicates to examine structural variants in hybrids genome wide and with a focus on previously mapped QTL regions.
2017-12-07 | GSE99481 | GEO
Project description:Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. In this study we aimed to understand and quantify the contribution of DNA methylation variation to heterosis in various traits of Arabidopsis. Therefore we created epigenetic hybrid lines from ddm1-derived epiRILs. We performed RNA sequencing on four epiHybrids and their parental lines (epiRILs & Col-wt) in duplicates/triplicates to understand gene expression changes in hybrids genome wide and with a focus on previously mapped QTL regions.
2017-12-07 | GSE99405 | GEO
Project description:Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. In this study we aimed to understand and quantify the contribution of DNA methylation variation to heterosis in various traits of Arabidopsis. Therefore we created epigenetic hybrid lines from ddm1-derived epiRILs. We performed whole genome bisulfite sequencing on four epiHybrids and their parental lines (epiRILs & Col-wt) in duplicates to understand methylome remodelling in hybrids genome wide and with a focus on previously mapped QTL regions.
2017-12-07 | GSE99404 | GEO
Project description:Genome-wide DNA methylation profiles from Arabidopsis epiRILs, epiHybrids and Colombia wild type
| PRJNA388579 | ENA
Project description:mRNA levels in Wild-type versus ddm1 Arabidopsis thaliana seedlings and bolting plants. Features found to be significantly enriched for DNA methylation were determined. This SuperSeries is composed of the SubSeries listed below.
2004-06-12 | GSE1354 | GEO