Project description:The phytopathogenic fungus Chrysoporthe cubensis is a relevant source of lignocellulolytic enzymes. This work aimed to compare the profile of lignocellulose- degrading proteins secreted by C. cubensis grown under semi-solid state fermentation using wheat bran and sugarcane bagasse. The proteins from the fungus extract grown in wheat bran (WBE) and sugarcane bagasse (SBE) were qualitative and quantitatively analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS). Label-free proteomic analysis of WBE and SBE showed that the fungus produced a spectrum of carbohydrate-active enzymes (CAZymes) with exclusive characteristics from each extract. While SBE resulted in an enzymatic profile directed towards the depolymerization of cellulose, the enzymes in WBE were more adaptable to the degradation of biomass rich in hemicellulose and other non-lignocellulosic polymers. Saccharification of alkaline pre-treated sugarcane bagasse with SBE promoted glucose release higher than commercial cocktails (8.11 g L -1 ), while WBE promoted the higher release of xylose (5.71 g L -1 ). Our results allowed an in-depth knowledge of the complex set of enzymes secreted by C. cubensis responsible for its high lignocellulolytic activity and still provided the identification of promising target proteins for biotechnological applications in the context of biorefinery.

2024-01-26 | PXD046075 | Pride

Chrysoporthe austroafricana strain:CMW6102

Project description:Chrysoporthe austroafricana Genome sequencing and assembly

| PRJNA420615 | ENA

Pseudoperonospora cubensis strain:MSU-1

Project description:Pseudoperonospora cubensis strain:MSU-1 Genome sequencing and assembly

| PRJNA80635 | ENA

Fusarium marasasianum strain:CMW 25512

Project description:Fusarium marasasianum strain:CMW 25512 Genome sequencing and assembly

| PRJNA773875 | ENA

Huntiella omanensis strain:CMW 11056

Project description:Huntiella omanensis strain:CMW 11056 Genome sequencing and assembly

| PRJNA263445 | ENA

Teratosphaeria zuluensis strain:CMW 17320

Project description:Teratosphaeria zuluensis strain:CMW 17320 Genome sequencing and assembly

| PRJNA544628 | ENA

Teratosphaeria gauchensis strain:CMW 17545

Project description:Teratosphaeria gauchensis strain:CMW 17545 Genome sequencing and assembly

| PRJNA544635 | ENA

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