Project description:Methicillin-resistant Staphylococcus aureus (MRSA) infections result in more than 200,000 hospitalizations and 10,000 deaths in the United States each year and remain an important medical challenge. To better understand the transcriptome of Staphylococcus aureus USA300 NRS384, a community-acquired MRSA strain, we have conducted an RNA-Seq experiment on WT samples.
Project description:In this dataset, we investigate the targets of degrasyn in the methicillin-sensitive S. aureus strain NCTC 8325. This includes data on enrichment studies and competition studies with conventional ABPP using a degrasyn-derived probe, competition studies with residue-specific proteomics using the isoDTB-ABPP method and global analysis of protein expression levels in response to degrasyn treatment.
Project description:To establish general differences between the protein expression in S. aureus strains, five methicillin sensitive S. aureus (MSSA) strains and five methicillin resistant S. aureus (MRSA) strains were compared both individually and as MSSA and MRSA groups in the absence of antibiotics. Proteins were compared by ultra-performance liquid chromatography-mass spectrometry.
