Dataset Information


Homo sapiens

ABSTRACT: Comparison of CD8+ memory T-cells from fresh and high density precultured PBMC.

ORGANISM(S): Homo sapiens  



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High-density preculture of PBMCs restores defective sensitivity of circulating CD8 T cells to virus- and tumor-derived antigens.

Wegner Julia J   Hackenberg Stephan S   Scholz Claus-Jürgen CJ   Chuvpilo Sergey S   Tyrsin Dmitry D   Matskevich Alexey A AA   Grigoleit Götz Ulrich GU   Stevanović Stefan S   Hünig Thomas T  

Blood 20150529 2

Peripheral blood mononuclear cells (PBMCs) are the only source of human lymphoid cells routinely available for immunomonitoring of T-cell responses to microbial and tumor-associated antigens. However, previous work in mice and humans had indicated that CD4 T cells transiently lose antigen sensitivity when cellular contacts are lost (eg, by entering the circulation). Using the simple and robust protocol for resetting T cells to original reactivity (RESTORE; ie, preculturing PBMCs for 2 days at a  ...[more]

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