Project description:Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.
Project description:Paramecium cells in stationary phase were treated for deciliation and total mRNA extracted at two time points (45 and 130 minutes) after deciliation. Keywords: Time course analysis of expression during reciliation
Project description:Paramecium cells possess a few thousand trichocysts (big secretory granules of 3~4 um long) docked at their surface and whose discharge in the external medium can be triggered using aminoethyldextran (a modified dextran positively charged) without damage to the cells. After discharge, new trichocysts are re-synthesized by the cells. We studied the evolution of the transcriptome during this synthesis.
