Genomics

Dataset Information

24

Synthetic construct


ABSTRACT: An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments

ORGANISM(S): synthetic construct  

PROVIDER: PRJNA268435 | ENA |

REPOSITORIES: ENA

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Publications

An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments.

Heyer Erin E EE   Ozadam Hakan H   Ricci Emiliano P EP   Cenik Can C   Moore Melissa J MJ  

Nucleic acids research 20141212 1


Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing. Here, we describe an optimized method of preparing strand-specific RNA deep sequencing libraries from small RNAs and variably sized RNA fragments obtained from ribonucleoprotein particle footprinting  ...[more]

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