Project description:Changes in Gene exporession after 8 weeks of PrimaVie Shilajit Supplementation were measured in vastus lateralis GeneChip® Human Transcriptome Array 2.0 was used to study the gene expression in human vastus lateralis after supplementation of PrimaVie Shilajit and identified distinct classes of up-regulated genes during this process.
Project description:Needle biopsies were obtained from the vastus lateralis muscle of 6 healthy, sedentary, 67±2.5 year-old males before and after 3 months of training. Keywords: other
Project description:To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition. Two-condition experiment, Vastus lateralis muscle biopsy post-expedition vs Vastus lateralis muscle biopsy pre-expedition. Five volunteers/climbers. Biological replicates: 1 pre-expedition sample, 1 post-expedition sample.
Project description:To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition.
Project description:To further unravel the potential molecular mechanisms involved in the loss of muscle function during an acute exacerbation, a cross-sectional microarray study was designed to compare the gene expression profile of the vastus lateralis muscle in patients with an acute COPD exacerbation and in stable COPD patients. Keywords: cross-sectional patient study
Project description:Development of a method to identify age-regulated expression trends in skeletql muscles and study the underlying molecular processes of the age_associated progressive changes in skeletal muscles. See abstract from associated manuscript below : Aging is characterized by progressive tissue degeneration and it is associated with genome-wide changes of mRNA expression profiles. Therefore, it is expected that age-regulated changes in expression profiles could describe the process of aging. We have developed a robust and unbiased methodology to identify age-regulated expression trends in cross-sectional data sets from healthy donors. Our analysis in four different tissues reveals that in brain cortex and Vastus lateralis muscles, two major age-associated expression inclinations were found, one during midlife and one in elderly. In kidney medulla and cortex, however, transcriptional changes were found only in elderly. In concurrence with increased degeneration in aged tissue, the elderly-associated expression changes are predominantly downwards. Our results suggest that a non-linear trend describes the aging process, and can be the result of inclinations of gene expression at two distinct age-positions affecting different molecular and cellular processes. RNA was extracted from vastus lateralis muscles. cDNA synthesis and labeling were preformed with the Illumina cDNA labeling kit.
Project description:We report RNA-seq data from the brown fat and vastus muscles of mice lacking IRF4 in brown fat (BATI4KO) vs floxed littermate controls
Project description:To further unravel the potential molecular mechanisms involved in the loss of muscle function during an acute exacerbation, a cross-sectional microarray study was designed to compare the gene expression profile of the vastus lateralis muscle in patients with an acute COPD exacerbation and in stable COPD patients. Keywords: cross-sectional patient study A cross-sectional microarray study was designed. The microarray screening was performed on a vastus lateralis biopsy obtained from 4 patients with an acute COPD exacerbation and 5 stable COPD patients. No replicates or dye-swaps were included.
