Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity.
Project description:We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus.
Project description:Gene expression was determined for Male accessory gland and testes in different members of the Anopheles gambiae complex, including An. gambiae (Savannah, MRA-762), An. coluzzii (Mopti) [2], An. arabiensis [3], An. merus [4] and An. quadriannalatus [5. All the mosquito stocks were obtained from the Malaria Research and Reference Reagent Resource Center (MR4) in Atlanta (https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx). References: 1. MR4 wild stocks information. Access Jenuary 2018. (Kisumu) https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx 2. MR4 wild stocks information. Access Jenuary 2018. (Mopti) https://www.beiresources.org/Catalog/BEIVectors/MRA-763.aspx 3. MR4 wild stocks information. Access Jenuary 2018. (arabiensis) https://www.beiresources.org/Catalog/BEIVectors/MRA-856.aspx 4. MR4 wild stocks information. Access Jenuary 2018. (merus) https://www.beiresources.org/Catalog/BEIVectors/MRA-1156.aspx 5. MR4 wild stocks information. Access Jenuary 2018. (quadriannulatus) https://www.beiresources.org/Catalog/BEIVectors/MRA-1155.aspx
Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity. After rearing in freshwater, separate pools of 18 h post-hatch larval instars of An. merus and An. coluzzii were briefly (6 h) exposed to one of six water salinities: 0%, 10%, 20%, 30%, 40%, or 50% the salinity of seawater. The last sample (G-50-RC) was split across 5 lanes for sequencing, resulting in total 10 fastq files associated with subparts G-50-RCa, G-50-RCb, G-50-RCc, G-50-RCd, G-50-RCe.
Project description:Field resistant Anopheles coluzzii were compared to the lab susceptible Anopheles coluzzii N'Gousso. The samples were collected in 2014 in Burkina Faso and show resistance to pyrethroid insecticides.
Project description:We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.
