Schizosaccharomyces pombe
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA270660 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Pcs1 and Mde4 are inner centromere proteins required to prevent merotelic kinetochore orientation
| PRJNA96477 | ENA
Project description:A key element for defining the centromere identity is the incorporation of a specific histone H3, CENP-A, known as Cnp1p in S. pombe. Previous studies have suggested that functional S. pombe centromeres lack nucleosome arrays and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are in fact positioned in regular intervals in the core of centromere 2, providing the first high resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore proteins cnp1, mis18, mis12, nuf2, mal2, overexpression of Cnp1p, or deletion of ams2. Bioinformatic analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence-bias in nucleosome positioning. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites for the GATA-like protein Ams2p, which participates in CENP-A incorporation. Keywords: Nucleosome Mapping Study Entire cnt regions and histone-related genes were tiled at 1-5 bp spacing using 60-mer probes.
2008-05-30 | E-GEOD-10742 | biostudies-arrayexpress
Project description:The metazoan nuclear periphery is involved in transcriptional regulation and chromatin organisation. To test whether this is also the case in the fission yeast Schizosaccharomyces pombe, we performed DamID experiments with two inner nuclear membrane (INM) proteins, Ima1 and Man1. The resulting map showed that about a third of the genome is associated with the nuclear periphery. We find that both INM proteins preferentially associate with lowly expressed genes, and are depleted from highly expressed genes. Further, intergenic regions of divergent gene pairs are more frequently associated with the periphery than convergent pairs, indicating that transcription points away from the periphery rather than toward it
2012-01-18 | GSE30476 | GEO
Project description:A key element for defining the centromere identity is the incorporation of a specific histone H3, CENP-A, known as Cnp1p in S. pombe. Previous studies have suggested that functional S. pombe centromeres lack nucleosome arrays and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are in fact positioned in regular intervals in the core of centromere 2, providing the first high resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore proteins cnp1, mis18, mis12, nuf2, mal2, overexpression of Cnp1p, or deletion of ams2. Bioinformatic analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence-bias in nucleosome positioning. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites for the GATA-like protein Ams2p, which participates in CENP-A incorporation. Keywords: Nucleosome Mapping Study
2008-05-30 | GSE10742 | GEO
Project description:Mediator promotes CENP-A incorporation at fission yeast centromeres [ChIP-seq]
| PRJNA156087 | ENA
Project description:Epigenetically induced paucity of histone H2A.Z stabilizes fission yeast ectopic centromeres
| PRJNA158255 | ENA
Project description:Genome wide map of H3K9me2 in 4 different strains of fission yeast Schizosaccharomyces pombe.
2019-01-03 | GSE119604 | GEO
Project description:DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast
| PRJNA189154 | ENA
Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains
2013-07-20 | GSE42848 | GEO
Project description:Meiotic recombination facilitates accurate pairing and faithful segregation of homologous chromosomes by forming physical connections (crossovers) between homologs. Developmentally programmed DNA double-strand breaks (DSBs) generated by Spo11 protein (Rec12 in fission yeast) initiate meiotic recombination. Until recently, attempts to address the basis of the highly non-random distribution of DSBs on a genome-wide scale have been limited to 0.1–1 kb resolution of DSB position. We have assessed individual DSB events across the Schizosaccharomyces pombe genome at near-nucleotide resolution by deep-sequencing the short oligonucleotides connected to Rec12 following DNA cleavage. The single oligonucleotide size-class generated by Rec12 allowed us to effectively analyze all break events. Our high-resolution DSB map shows that the influence of underlying nucleotide sequence and chromosomal architecture differs in multiple ways from that in budding yeast. Rec12 action is not strongly restricted to nucleosome-depleted regions but is nevertheless spatially biased with respect to chromatin structure. Furthermore, we find strong evidence across the genome for differential DSB repair previously predicted to account for crossover invariance (constant cM/kb in spite of DSB hotspots). Our genome-wide analyses demonstrate evolutionarily fluid factors contributing to crossover initiation and its regulation.
2014-06-25 | GSE49977 | GEO