Project description:Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation.

2021-09-09 | PXD021370 | Pride

Ixodes ricinus

Project description:Ixodes ricinus Transcriptome or Gene expression

| PRJNA595586 | ENA

Ixodes ricinus

Project description:Ixodes ricinus Transcriptome or Gene expression

| PRJNA177622 | ENA

Ixodes ricinus

Project description:midgut transcriptome shotgun assembly from unfed Ixodes ricinus females

| PRJNA270980 | ENA

Ixodes ricinus

Project description:TBEV effect on Ixodes ricinus salivary transcriptome