Project description:This SuperSeries is composed of the following subset Series: GSE16140: Transcriptome analysis of rice (Oryza sativa cv.TW16) in relation to infection with rice tungro spherical virus (RTSV) GSE16141: Transcriptome analysis of rice (Oryza sativa cv. Taichung Native 1) in relation to infection with RTSV Refer to individual Series
Project description:Rice stripe virus (RSV) is one of the major virus diseases of rice in East Asia. Rice plants infected with RSV usually show symptoms such as chlorotic leaf stripes, weakness and senescence of leaves, and dwarfism. In order to characterize the host response to RSV infection at the gene expression level, the changes in transcriptome profiles of RSV-infected rice were monitored at three, six, nine, twelve, and fifteen days after inoculation by a rice oligomicroarray. The microarray data indicated that 1. transcription, translation and protein processing machineries were activated, 2. chloroplasts were disintegrated, and mitochondrion function was activated, 3. genes for transporters and cell wall synthesis were suppressed, and 4. the expression levels of pathogenesis-related genes were changed by RSV infection. Concurrent observation of symptom development, virus accumulation and transcriptome profiles in RSV-infected plants indicates that RSV symptoms are caused by unbalanced activities of organelles, suppression of cell elongation, and uncontrolled water transport, while translation activity of host cells may be increased in correlation with RSV propagation. Keywords: time course, virus infection, disease response
Project description:During the pathogenesis of virus disease, lots of changes occur in plant hosts including the altered gene expression profiles. Rice stripe virus (RSV) is the type member of Tenuivirus, transmitted by Laodelphax striatellus in a circulative propagative man To investigate the molecular differences in two japonica rice genotypes WuYun3 and KT95-418 in response to RSV infection, Affymetrix rice genome array were used to analyze and compare their transcriptional profiles. Keywords: disease state analysis
Project description:During the pathogenesis of virus disease, lots of changes occur in plant hosts including the altered gene expression profiles. Rice stripe virus (RSV) is the type member of Tenuivirus, transmitted by Laodelphax striatellus in a circulative propagative man; To investigate the molecular differences in two japonica rice genotypes WuYun3 and KT95-418 in response to RSV infection, Affymetrix rice genome array were used to analyze and compare their transcriptional profiles. Experiment Overall Design: Nine-day old 20 rice seedlings were exposed to approximately 100 viruliferous small brown planthoppers (Laodelphax striatellus Fall) for two days to be innoculated by RSV. Then they were transplanted in insect-free greenhouse at 25±3C with a daily photope
Project description:Rice stripe virus (RSV) is one of the major virus diseases of rice in East Asia. Rice plants infected with RSV usually show symptoms such as chlorotic leaf stripes, weakness and senescence of leaves, and dwarfism. In order to characterize the host response to RSV infection at the gene expression level, the changes in transcriptome profiles of RSV-infected rice were monitored at three, six, nine, twelve, and fifteen days after inoculation by a rice oligomicroarray. The microarray data indicated that 1. transcription, translation and protein processing machineries were activated, 2. chloroplasts were disintegrated, and mitochondrion function was activated, 3. genes for transporters and cell wall synthesis were suppressed, and 4. the expression levels of pathogenesis-related genes were changed by RSV infection. Concurrent observation of symptom development, virus accumulation and transcriptome profiles in RSV-infected plants indicates that RSV symptoms are caused by unbalanced activities of organelles, suppression of cell elongation, and uncontrolled water transport, while translation activity of host cells may be increased in correlation with RSV propagation. Keywords: time course, virus infection, disease response Comparison between RSV- and mock-infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested; 4 time points (3, 6, 9, 12 days after inoculation (DAI)). Biological replicate: 1 control, 1 infected, independently grown and harvested; 1 time point (15 DAI). 1 sample derived from 5 plants grown under the same conditons.
Project description:The rice blast disease, caused by Magnaporthe oryzae , devastates cultivated rice (Oryza sativa L.), resulting in extensive global crop loss. We employed a label-free quantitative proteomics approach to discover novel proteins associated with M. oryzae pathogenicity and rice defense. We identified 990 significantly modulated proteins in rice leaves including various pattern recognition receptors (PRRs) and pathogenesis-related (PR) proteins that were induced in response to M. oryzae inoculation. Additionally, 123 M. oryzae proteins were also identified and screened for their cell death-inducing activity by an in-silico approach. Among these, we found a novel protein MoXYL1 (endo-1,4-beta-xylanase) protein, which induces cell death in Nicotiana benthamiana leaves. Transgenic rice plants (PDUF26::MoXYL1) expressing MoXYL1 derived by rice domain of unknown function protein 26 (DUF26) promoter exhibited resistance against the M. oryzae and Cochliobolus miyabeanus and enhanced expression of pathogen-responsive genes and hormone-related genes. Furthermore, the application of data-independent acquisition (DIA) mass spectrometry (MS)-based proteomics on these transgenic rice plants revealed 1,833 significantly modulated proteins in response to M. oryzae, with 219 and 410 proteins responsive to MoXYL1 and M. oryzae, respectively. Based on these results, we propose a signaling network model induced by MoXYL1 and M. oryzae. In summary, our findings highlight the crucial role of MoXYL1 in rice innate immunity against M. oryzae and its potential to enhance rice disease resistance.
Project description:This experiment probed for the presence of known Arabidopsis and rice microRNAs in total RNA samples derived from species representative of the major groups of land plants: Eudicots (Arabidopsis thaliana, Nicotiana benthamiana), monocots (Oryza satica, Triticum aestivum), magnoliids (Liriodendron tulipifera), gymnosperms (Pinus resinosa), ferns (Ceratopteris thalictroides), lycopods (Selaginella uncinata), and mosses (Polytrichum juniperinum). In most cases two technical or biological replicates were performed.
Project description:Os02g31890 encodes a dehydration-responsive transcription factor (named ´ARID´) from rice (Oryza sativa, cv. Dongjin). Expression profiling was performed 90 min after the start of dehydration stress in roots of Oryza sativa wild-type plants (cv. Dongjin) and a knock-out (i.e. arid) mutant.
Project description:To investigate the relative effects of drought and heat on the rice (Oryza sativa) transcriptome, we subjected rice seedlings to 3 levels of long-term drought or 2 levels of short-term heat stock