Project description:Petal is not only the target of selection by horticulturalists to enhance the ornamental value of plants but also emerged as a unique model system for plant organogenesis studies. It is known that three major groups of pigments, betalains, carotenoids and anthocyanins, are responsible for the attractive natural display of flower colors. While carotenoids and betalains generally yield yellow or red colors, anthocyanins confer a diverse range of color from orange to red to violet and blue. In this study, we collected 11 species (Erysimum cheiri, Malcolmia maritime, Brassica oleracea, Raphanus sativus, Orychophragmus violaceus, Eruca sativa, Orychophragmus violaceus, Iberis amara, Aubrieta x cultorum, Lobularia maritime, Matthiola incana) belong to different tribe in Brassicaceae family with varied flower color and performed petal transcriptome analysis. de novo transcriptome assembly showed that average length of the contigs varied from 631bp in O. violaceus to 1212bp in Matthiola incana which indicated that the complexity of the genomes are different much. Protein homology between these species and those sequenced species in Brassicaceae family are consistent with the known phylogenetic relationships. However, O. violaceus has closer relationships with Sisymbrium irio than expected Brassica species. Clustering analysis of genes in anthocyanin and carotenoids synthesis pathway indicated that while silence or low expression of CCD4 (Carotenoid Cleavage Dioxygenase 4) leading to the yellow color formation in different species, purple or red color variation might result from different genes expression variation. These results not only provide transcriptome data for petal development study but also provide useful information for Brassica flower improvement for ornamental purpose.
Project description:Gerbera delavayi Franch. endemic to southwest China, is a rare fiber plant. In this study, the leaves of G. delavayi were sequenced based on Illumina Hi-Seq2500. The results showed that 108694 unigenes were found. N50 was 593.90bp, and the mean length was 912bp. By comparing with Nr and Swiss-prot database, 40915 unigenes were annotated, and 67779 unigenes were unannotated. In addition, 30 unigenes had homology with Ces family, 20 unigenes had homology with Cls family, and 11 unigenes had homology with SuSy. 11369 unigenes were assigned to 25 categories with COG database, and 21378 unigenes were divided into 52 GO terms. Function annotation against KEGG database obtained 8087 unigenes and 118 pathways. 47 unigenes were found at “phenylpropanoid biosynthesis” pathway. Furthermore, 4908 unigenes contained 5179 SSRs, 1 SSR occurred every 12.46kb. The largest number of SSR type was mono-nucleotide repeat, and its frequency was 54.37%; the next was di-nucleotide repeat and tri-nucleotide repeat, with the frequencies of 22.90% and 21.70%, respectively. These results greatly enriched the genetic information of G. delavayi, and provided basic data for genetic breeding and exploitation of this unique plant resource.
Project description:We used high-throughput sequencing to identify conserved and nonconserved miRNAs and other short RNAs in Paeonia ostii under control and copper stressed condition. 102 previously known plant miRNAs were identified and classified into 89 families according to their gene sequence identity. Some miRNAs were highly conserved in the plant kingdom suggesting that these miRNA play important and conserved roles. Combined our transcriptome sequencing data of Paeonia ostii under same conditions, 34 novel potential miRNAs were identified. The potential targets of the identified known and novel miRNAs were also predicted based on sequence homology search. Comparing the two libraries, it was observed that 12 conserved miRNAs and 18 novel miRNAs showed significantly changes in response to copper stress. Some of the new identified potential miRNAs might be involved in Paeonia ostii-specific regulating mechanisms under copper stress. These results provide a framework for further analysis of miRNAs and their role in regulating Paeonia ostii response to copper stress.