Project description:Sea cucumber, Apostichopus japonicus is a very important species for aquaculture, and its behavior and physiology can respond to the initial change in salinity. It is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity In this study, RNA-seq provided a general overview of the gene expression profiles of the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environment. Overall design: Sea cucumbers were acclimated in tanks containing aerated sand-filtered seawater (salinity 30%, pH 8.0) at 15 ± 0.5°C for one week before use and were fed once each day at 8:00 am during this period. The animals were then assigned randomly to three groups (20 individuals in each group). One group of sea cucumbers was maintained as the control group with normal salinity (SD30) of around 30, whereas the other two groups were kept in sea water where the salinity was gradually increased and decreased to high salinity (SD40) of around 40 and low salinity (SD20) of around 20 at a rate of 2.5 per 6 h.
Project description:Natural DNA methylation variation was examined in a population of wild Lotus japonicus accessions from Japan Overall design: Whole genome DNA methylation data for 18 wild Lotus japonicus accessions (two with replicates) grown at 20C
Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.