Project description:Transcriptomic changes during haustoria formation in the parasitic plant Phtheirospermum japonicum. We performed RNAseq on Phtheirospermum japonicum seedling infecting and not infecting over 5 time points during haustoria formation for the control treatment and over 3 time points for the ammonium nitrate and 6-Benzylaminipurine. We investigated the transcriptomic changes during haustoria formation and how this is affected in the transcritpome level by nitrate and cytokinin treatment.
Project description:47,984 unigenes with putative coding regions with at least 50 amino acids were selected from whole transcriptome (SAMN03271814) of Phtheirospermum japonicum Microarray slides were hybridized with labeled cRNA from roots of 2-week-old Phtheirospermum japonicum plants growing axenically and treated with haustoria-inducer DMBQ (10 µM) for 0, 0.5, 1, 3, 6, 12, 24 and 48 h
