Project description:Purpose: To profile the effect of the Maf1 knockout on RNA polymerase II and RNA polymerase III transcriptomes in white adipose tissue Methods: Epididymal adipose tissue was harvested from overnight fasted 22-24 week old male mice that had been fed ad libitum on a chow diet. Total RNA was prepared from three biologically independent samples per genotype and subject to single-end directional RNA sequencing on the Illumina HiSeq platform. Sequence reads that passed quality filters were aligned against the Mm9 Mouse genome assembly using GSNAP and uniquely mapped reads were counted using HTseq-counts.
Project description:We studied adipose tissue from wild type mice, kinin B1 receptor knockout mice (B1KO), and B1KO mice with rescued expression of kinin B1 receptor selectively in fat. We used microarrays to detail the gene expression profile of the epididymal fat of these mice.
Project description:The goal of this study is to identify the cistrome of the transcriptional repressor Rev-erb alpha in epididymal white adipose tissue. Performing Rev-erb alpha ChIP-seq on epididymal white adipose tissue from wildtype mice at 5PM when Rev-erb alpha protein level peaks in wild type (WT) mice, we were able to globally determine the genomic regions undergoing Rev-erb alpha-dependent de-repression. Examination of Rev-erb alpha binding in epididymal white adipose tissue.
Project description:The goal of this study is to identify the cistrome of the transcriptional repressor Rev-erb alpha in epididymal white adipose tissue. Performing Rev-erb alpha ChIP-seq on epididymal white adipose tissue from wildtype mice at 5PM when Rev-erb alpha protein level peaks in wild type (WT) mice, we were able to globally determine the genomic regions undergoing Rev-erb alpha-dependent de-repression.
Project description:We attempted to analyze the effect of adipocyte-specific knockout of Prmt1 in energy homeostasis in mice. By analyzing the transcriptomics of epididymal primary adipocytes from male mice with wild type and adipocyte-specific Prmt1 knockout mice, we provide the insight into the collective roles of Prmt1 in epididymal white adipose tissues.
Project description:We studied adipose tissue from wild type mice, kinin B1 receptor knockout mice (B1KO), and B1KO mice with rescued expression of kinin B1 receptor selectively in fat. We used microarrays to detail the gene expression profile of the epididymal fat of these mice. Three-month-old mice were killed and tissue was collected for RNA processing and microarray analysis.
Project description:We attempted to analyze the effect of adipocyte-specific knockout of Crtc2 in in aging. By analyzing the transcriptomics of epididymal primary adipocytes from male mice with wild type and adipocyte-specific Crtc2 knockout mice in response to aging, we provide the insight into the collective roles of Crtc2 in epididymal white adipose tissues upon aging.
Project description:Adipose stem and progenitor cells, defined as live CD45- CD31- Ter119- CD140a+ Sca1+ PDPN+, were sorted from the epididymal adipose tissue of three wild type 12 week old male mice for transcriptional analysis.
Project description:Compare miRNA expression profiles in epididymal white adipose tissue (WAT), interscapular brown adipose tissue (BAT) and skeletal muscle from wild-type C57BL/6J mice
Project description:To investigate glucose intolerance and white adipocyte hypertrophy shown in ZFP251 knockout (KO) mice, We performed gene expression profiling analysis using data obtained from the RNA-seq of epididymal white adipose tissue (eWAT).
