Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction. Two-condition experiment, uninfected chicken embryo lung cells vs. infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 infected experimental sample at each 1dpi, 3dpi, 5dpi, and 7 dpi.
Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction.
Project description:Host gene expression of chicken embryo lung cells infected an infectious laryngotracheitis virus (ILTV) vaccine strain comparing control uninfected lung cells. Goal was to identify the changes of host gene expression by ILTV vaccine infection.
Project description:Cell transformation by the Src tyrosine kinase is characterized by extensive changes in gene expression. To describe these changes, investigators have relied extensively on the study of immortalized rodent cell lines or heterogeneous tumor samples that limit the identification of differentially expressed genes or may not represent the full spectrum of biological processes regulated during transformation. In this study, we took advantage of transformation-deficient and temperature sensitive mutants of the Rous sarcoma virus to characterize the patterns of gene expression in two types of primary cells, namely chicken embryo fibroblasts (CEF) and chicken neuro-retinal (CNR) cells. Keywords: viral transformation of primary cells, transformation, transformation deficient mutant, temperature sensitive mutant, v-Src Chicken embryo fibroblasts (CEF) were infected with the wild-type strain Schmidt-Ruppin A RSV or non-transforming strain NY315 RSV or the non-transforming control virus RCASBP(A) to assess genes involved in v-Src-dependent transformation of CEF. Chicken embryo fibroblasts (CEF) were infected with the temperature sensitve strain NY72-4 RSV and cultured either at non-permissive temperature (41.5M-KM-^ZC) or permissive temperature (37M-KM-^ZC) to assess genes involved in v-Src-dependent transformation of CEF. Chicken neuroretina cells (CNR) were infected with the temperature sensitve strain NY72-4 RSV and cultured either at non-permissive temperature (41.5M-KM-^ZC) or permissive temperature (37M-KM-^ZC) to assess genes involved in v-Src-dependent transformation of CNR and compared to CEF.
Project description:Fowlpox virus (here as FP9, a plaque-purified, high passage-attenuated derivative) effectively suppresses induction of the âinnateâ immune responses, notably the Type I interferon system, of the permissive host (chicken). Despite the extensive usage of fowlpox virus as a recombinant vaccine vector in chickens, its immunomodulatory mechanisms remain largely unknown. In this study, a transcriptomic analysis using the Affymetrix GeneChip chicken genome array was performed at the host gene transcription level at 4, 8, 16 and 24 hours post-infection of mock treated and FP9-infected (MOI=5, 2h) chicken embryo fibroblasts (CEF). The experiment was performed in triplicate with three different batches of CEFs. Because fowlpox virus is capable of expressing antigens in mammalian cells, these studies in chicken cells form a baseline for subsequent study of immunomodulation of mammalian innate immune responses.
Project description:Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).
Project description:Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).
