Project description:A Platichthys flesus cDNA microarray (GENIPOL) comprised of 12,700 clones, was used to determine and characterize gene expression profiles of flounders (Platichthys flesus) exposed to polybrominated diphenyl ethers (pentamix).
Project description:A Platichthys flesus cDNA microarray (GENIPOL) comprised of 12,700 clones, was used to determine and characterize gene expression profiles of environmentally sampled flounders from sites of different pollution status in Northern Europe.
Project description:Proteomics of livers from Platichthys flesus caught in the Canche estuary but having undergone a 1-month encagement in three estuarine sites for describing molecular players in response to encagement and environmental stresses.
Project description:Using the GENIPOL flounder cDNA microarray, we assessed the temporal transcriptomic responses of Platichthys flesus to model toxicants over a 16 day timespan. Immature fish were treated by intraperitoneal injection with cadmium chloride (50 micrograms/kg in saline), 3-methylcholanthrene (25mg/kg in olive oil), Aroclor 1254 (50mg/kg in olive oil), tert-butyl-hydroperoxide (5mg/kg in saline), lindane (25 mg/kg in olive oil), perfluoro-octanoic acid (100mg/kg in olive oil), olive oil or saline (0.9%). Hepatic gene expression changes were determined 1,2,4,8 and 16 days post-injection in comparison with time-matched carrier controls.
