Project description:Mice were immunized with either formalin fixed Influenza A/PR/8/34 (Killed PR8), the 2006-2007 seasonal influenza vaccine, the 2007-2008 seasonal influenza vaccine, a sublethal infection (live PR8) or mock immunized (PBS). Array data was used to distinguish the immunogens from each other and predict which of the three inactivated vaccines would be protective against A/PR/8/34 challenge.
Project description:Mouse lung RNAseq after infection with Influenza A virus (H1N1, PR/8/34, mouse-adapted) and Streptococcus pneumoniae (serotype 19F, strain BHN 100) Results: Differentially expressed genes were observed after single and co-infection Project: COST_mouse_2021_lung
Project description:Mouse blood transcriptome after infection with Influenza A virus (H1N1, PR/8/34, mouse-adapted) and Streptococcus pneumoniae (serotype 19F, strain BHN 100) Results: Differentially expressed genes were observed after single and co-infection Project: COST_mouse_2021
Project description:Mice were immunized with either formalin fixed Influenza A/PR/8/34 (Killed PR8), the 2006-2007 seasonal influenza vaccine, the 2007-2008 seasonal influenza vaccine, a sublethal infection (live PR8) or mock immunized (PBS). Array data was used to distinguish the immunogens from each other and predict which of the three inactivated vaccines would be protective against A/PR/8/34 challenge. two replicates of each peptide was printed on 1 CIM_10kv3 peptide microarray. One microarray were tested for each sample. Image was qualified using in-house metrics for quality assurance.
Project description:This SuperSeries is composed of the following subset Series: GSE35265: Analysis of global gene expression profiles of hPAF1 deficiency on unstimulated A549 cells GSE35266: Analysis of global gene expression profiles of hPAF1 deficient A549 cells during infection with H1N1 influenza A virus or vesicular stomatitis virus (VSV) GSE35267: Analysis of global gene expression profiles of hPAF1 deficient A549 cells during stimulation with PR8/?NS1 influenza virus, IFN?1 or Poly(I:C) Refer to individual Series
Project description:Untransfected (no siRNA), control siRNA or XRN2 siRNA treated A549 cells were analyzed for gene expression differences under basal conditions
Project description:Antiviral responses must be regulated to rapidly defend against infection while minimizing inflammatory damage, but the mechanisms for establishing the magnitude of response within an infected cell are not well understood. miRNAs are small non-coding RNAs that negatively regulate protein levels by binding target sequences on their cognate mRNA. We profiled microRNA expression in the lungs of mice infected for 24 h with Influenza A/PR/8/34 to identify microRNAs that may regulate host response to influenza infection. Mice were infected intranasally with 10e5 pfu Influenza A/PR/8/34. Lungs were isolated after 24 hours and total RNA extracted using Trizol. Equivalent quantities of RNA from 3 infected mice were pooled.
Project description:We report the whole genome sequencing of human bronchial cells infected with influenza A (PR/8/34) for 8 or 24 h. Examination influenza virus-specific human gene profiling at early or late stage of viral infection.
