ABSTRACT: Expression data from the complex I mutant gas-1 (fc21) C. elegans worms in comparison to wildtype N2 Bristol worm and under the inluence of antioxidant drug treatment
Project description:We studied genome-wide expression profiles of C. elegans young adult worms, complex I mutant gas-1(fc21) compare to the wild type N2 Bristol worm and the effect of different antioxidant drug treatment on the gene expression profile in gas-1(fc21).
Project description:We studied genome-wide expression profiles of C. elegans young adult worms, complex I mutant gas-1(fc21) compare to the wild type N2 Bristol worm and the effect of combination drug treatment on the gene expression profile in gas-1(fc21).
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 rsd-2 and Bristol N2 rsd-6 single mutants, compared to late-generation strains at 25°C and 20°C
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 prg-1 and Bristol N2 prg-1; daf-2 double mutant, compared to late-generation strains. A seven chip study using total RNA recovered from three separate alleles of Caenorhabditis elegans Bristol N2 prg-1 and four separate allelic combinations of a double mutant strain, Bristol N2 prg-1; daf-2 , in which prg-1 (pk2298) and prg-1 (tm872) are combined with one of three daf-2 alleles (e1368, 1370, m41).
Project description:Expression data from the complex I mutant gas-1 (fc21) C. elegans worms in comparison to wildtype N2 Bristol worm and under the inluence combination drug therapy
Project description:Investigation of whole genome gene expression level changes in early generation Caenorhabditis elegans Bristol N2 rsd-2 and Bristol N2 rsd-6 single mutants, compared to late-generation strains at 25°C and 20°C A six chip study using total RNA recovered from two separate alleles of Caenorhabditis elegans Bristol N2 rsd-2 and one allele Bristol N2 rsd-6 , in which the effects of transgeneration aging are studied in early generation animals and late generation animals at the restrictive temperature of 25°C and the permissive temperature, 20°C.
Project description:Transcriptional profiling of two C. elegans mutants of dnj-14, the worm homolog of cysteine string protein (CSP), compared to two control strains N2 (Bristol) and CZ1200. CSP is a neuroprotective protein and the C. elegans mutants, tm3223 and ok237, are used to model the neurodegenerative disease, adult onset neuronal ceroid lipofucinosis (ANCL), which can occur as a result of mutations in the human CSP gene, DNAJC5. It is hoped that transcriptional profiling of these mutants might help to identify novel therapeutic targets for neurodegerative diseases such as ANCL.
Project description:Transcriptome profiling of three models with impaired insulin/IGF1 signaling. 1. Deep sequencing of endogenous mRNA from Caenorhabditis elegans N2 var. Bristol (wildtype) and daf-2(e1370) mutant; 2. Deep sequencing of endogenous mRNA from murine embryonic fibroblasts (MEF) wildtype and irs1-/- knockout; 3. Deep sequencing of endogenous mRNA from murine embryoinic fibroblast (MEF) insr+/- -lox and insr+/- knockout 14 samples examined: C. elegans N2 var. Bristol (wildtype) vs. daf-2(e1370) mutant; MEF wildtype vs. irs1-/- knockout; MEF insr+/- -lox vs. insr +/- knockout
Project description:Cocoa protein content is a very interesting source for isolation of antioxidant bio-peptides, which can be used for the prevention of age-related diseases. We use microarrays to study the global genome expression of C. elegans fed with a peptide (13L) isolated from cocoa. Wild type strain N2 of C. elegans was fed with 1 µg/mL of 13L peptide or in Nematode Growth medium (MGM, control fed) until reach young adult stage. Worm population were age-synchronized. RNA was isolated from each populations (control and treated) using RNAasy Kit (Qiagen) and hybridizated on Affymetrix microarrays.
