Project description:Different microRNA Alterations Contribute to Diverse Outcomes Following EV71 and CA16 Infections: Insights from High-Throughput Sequencing in Peripheral Blood Mononuclear Cells of Rhesus Monkey
Project description:Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are the predominant etiological agents of hand, foot, and mouth disease (HFMD) and both belong to the human enterovirus A species of the Picornaviridae family. These viruses share similar genetic homology, although the clinical manifestations of HFMD caused by the two viruses have some discrepancies. Furthermore, the underlying mechanisms leading to these differences remain unclear. microRNAs (miRNAs) participate in numerous biological or pathological processes, including host responses to viral infections, by targeting messenger RNAs (mRNAs) for translational repression or degradation. Here, we focused on differences in miRNA expression patterns in peripheral blood mononuclear cells (PBMCs) of rhesus monkeys infected with EV71 or CA16 at different time points using high-throughput sequencing technology. For the first time, this study demonstrated that EV71 and CA16 infection result in specific miRNA expression patterns in PBMCs.
Project description:The present work focuses on global gene expression of peripheral blood mononuclear cells in rhesus monkey infants with HFMD induced by CA16 infection at different time points. Genome-wide expression and analysis were performed by using Agilent whole genome microarrays and established bioinformatics tools. 931 signiM-oM-,M-^Acantly differentially expressed genes associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, by mapping genes related to immune system process on KEGG pathways, the predominance of inflammation mediate by chemokine and cytokine signaling pathway and interleukin signaling pathway emerged. Ultimately, co-expression genes and their networks were analysised. Coxsackievirus A16 infection induced gene expression in rhesus monkey peripheral blood mononuclear cells was measured at 7-1st dpi, 3-2nd dpi, 7-2nd dpi, 14-2nd dpi. Three samples from two experiment donors and one control donor were performed at each time. To exclude the individual difference, mix the two experiment samples at each time, and mix the two control samples.
